Product Information
The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from 1-5µg ofpreviously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.
Note that for input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).
- This product is related to the following categories:
- RNA Library Prep for Illumina,
- Next Generation Sequencing Library Preparation Products
Reagents Supplied
The following reagents are supplied with this product:
Properties & Usage
Materials Required but not Supplied
- 96-well 0.2 ml PCR Plates and Microseal® 'B' Adhesive Sealer (BioRad MSB-1001) or 0.2 ml RNase-free tube.
- Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
- Thermal cycler or heat block
- Bioanalyzer® (Agilent Technologies, Inc.) or similar instrument and consumables.
Advantages and Features
Application Features
Isolation of poly(A)+ RNA transcript from Total RNA for RNA library preparation and sequencing.
Protocols, Manuals & Usage
Protocols
- Where can I find protocols for using NEBNext®RNA Library Prep reagents?
Manuals
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
- manualE7490
Usage Guidelines
- Getting Started with RNA-Sequencing (RNA-Seq)
FAQs & Troubleshooting
FAQs
- Can I isolate poly(A)+ mRNA directly from tissue lysis using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490)?
- Do I need two rounds of binding?
- Can I use the Oligo (dT)25 Magnetic Beads (NEB# S1419S) to isolate poly(A)+ mRNA for Next Generation Sequence applications?
Citations & Technical Literature
Citations
NEBNext Product Citations Search Tool
Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.
Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
- E7490S_L_v1
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
- E7490G_v0_10010544
- E7490L_v0_10012676
- E7490S_v0_10011311
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- E7490L_v1_10042215
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- E7490G_v0_10042203
- E7490L_v1_10059821
- E7490S_v1_10042209
- E7490L_v1_10059927
- E7490L_v1_10061944
- E7490G_v0_10063351
- E7490L_v1_10054407
- E7490S_v1_10064141
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- E7490S_v1_10054401
- E7490G_v0_10070797
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- E7490L_v1_10061867
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- E7490L_v1_10082500
- E7490G_v0_10054395
- E7490S_v1_10084451
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- E7490L_v1_10087559
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- E7490L_v1_10091923
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- E7490S_v1_10071730
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- E7490G_v0_10129243
- E7490L_v1_10129757
- E7490S_v1_10129439
- E7490G_v0_10131814
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- E7490S_v1_10135473
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- E7490S_v1_10158079
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- E7490S_v1_10136053
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- E7490L_v1_10171851
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- E7490L_v1_10175963
- E7490L_v1_10136047
- E7490S_v1_10179077
- E7490G_v1_10182014
- E7490L_v1_10180870
- E7490S_v1_10182922
- E7490L_v1_10184197
- E7490S_v1_10186421
- E7490S_v1_10190960
- E7490L_v1_10159007
- E7490G_v1_10136033
- E7490S_v1_10159013
- E7490L_v1_10198461
- E7490S_v1_10200967
- E7490L_v1_10208853
- E7490L_v1_10207136
- E7490S_v1_10187013
- E7490G_v1_10187001
- E7490L_v1_10187007
- E7490L_v1_10216916
- E7490S_v1_10209211
- E7490G_v1_10209202
- E7490L_v1_10209208
- E7490G_v1_10236191
- E7490L_v1_10236249
- E7490S_v1_10236194
- E7490S_v1_10242511
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
-
NEBNext RNA Binding Buffer (2X)
-
NEBNext Wash Buffer
-
Nuclease-free Water
-
NEBNext Tris Buffer
-
NEBNext Oligo d(T)25 Beads
-
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
This product is for research use only, not for therapeutic use, and for use only in nucleic acid purification as part of sample preparation prior to sequencing.
